Survivability Analysis of the Iridium Low Earth Orbit Satellite Network

This thesis evaluates the survivability of the proposed Iridium Low Earth Orbit (LEO) Satellite Network. In addition to the complete Iridium constellation, three degraded Iridium constellations are analyzed. This analysis occurs via the use of simulation models, which are developed to use three dynamic routing algorithms over three loading levels. The Iridium network models use a common set of operating assumptions and system environments. The constellation survivability was determined by comparing packet rejection rates, hop\u27 counts, and average end to end delay performance between the various network scenarios. It was concluded that, based on the established scenarios, the proposed Iridium constellation was highly survivable. Even with only 45 percent of its satellites functioning (modeled with 36 failed Iridium satellites), the average packet delays were never greater than 178 milliseconds (msec), well within the real time packet delivery constraint of 400 msec. As a result, while additional research is necessary, Iridium has demonstrated the network robustness that is required within the military communications environment

Noncanonical DNA Motifs as Transactivation Targets by Wild Type and Mutant p53

Sequence-specific binding by the human p53 master regulator is critical to its tumor suppressor activity in response to environmental stresses. p53 binds as a tetramer to two decameric half-sites separated by 0–13 nucleotides (nt), originally defined by the consensus RRRCWWGYYY (n = 0–13) RRRCWWGYYY. To better understand the role of sequence, organization, and level of p53 on transactivation at target response elements (REs) by wild type (WT) and mutant p53, we deconstructed the functional p53 canonical consensus sequence using budding yeast and human cell systems. Contrary to early reports on binding in vitro, small increases in distance between decamer half-sites greatly reduces p53 transactivation, as demonstrated for the natural TIGER RE. This was confirmed with human cell extracts using a newly developed, semi–in vitro microsphere binding assay. These results contrast with the synergistic increase in transactivation from a pair of weak, full-site REs in the MDM2 promoter that are separated by an evolutionary conserved 17 bp spacer. Surprisingly, there can be substantial transactivation at noncanonical ½-(a single decamer) and ¾-sites, some of which were originally classified as biologically relevant canonical consensus sequences including PIDD and Apaf-1. p53 family members p63 and p73 yielded similar results. Efficient transactivation from noncanonical elements requires tetrameric p53, and the presence of the carboxy terminal, non-specific DNA binding domain enhanced transactivation from noncanonical sequences. Our findings demonstrate that RE sequence, organization, and level of p53 can strongly impact p53-mediated transactivation, thereby changing the view of what constitutes a functional p53 target. Importantly, inclusion of ½- and ¾-site REs greatly expands the p53 master regulatory network

The structure of the weak neutral current: an analysis of the hadronic energy distribution from neutrino and antineutrino interactions

An analysis is presented of the distribution of hadronic energy in neutrino and antineutrino neutral current interactions occurring in BEBC, filled with a neon-hydrogen mixture and exposed to the CERN-SPS narrow-band neutrino beam. This shows that the contributions by scalar or pseudo-scalar forms of the interaction are consistent with zero and pure V, A and V+A are excluded; there is good agreement with the Weinberg-Salam model. (10 refs)